[“Understanding Metabolism

The failure of store depletion by arachidonic acid to activate capacitative calcium entry may be due to the previously documented ability of arachidonic acid to inhibit capacitative calcium entry. Clearly, additional work will be needed to understand the varied patterns and mechanisms that control the biologically important phenomenon known as ioscillations.AcknowledgmentsWe are grateful to Elizabeth Murphy and Jerry Yakel who read the manuscript and provided helpful comments.jbc.M When a correction for this article is posted Click here to choose from all of JBCs email alerts This article cites references, of which can be accessed free at http:www.jbc.orgcontent.full.htmlreflist Downloadedfromhttp: www.jbc.orgbyguestonSeptember, To investigate the molecular basis of oxidative stressinduced T cell hyporesponsiveness, we have developed an in vitro system in which T lymphocytes are rendered hyporesponsive by coculture with oxygen radicalproducing activated neutrophils.We have observed a direct correlation between the level of T cell hyporesponsiveness induced and the concentration of reactive oxygen species produced.The pattern of tyrosinephosphorylated proteins was profoundly altered in hyporesponsive as compared with normal T cells.T lymphocytes isolated from patients affected with human pathologies such as cancer, AIDS, rheumatoid arthritis. The costs of publication of this article were defrayed in part by the payment of page charges.This article must therefore be hereby marked advertisement in accordance with U.In T lymphocytes from AIDS patients, plck has a decreased activity that probably results from a conformational change due to an altered intracellular redox potential. T lymphocytes isolated from patients affected with certain cancers have decreased expression levels of TCR, and macrophages from tumorbearing mice can induce such partial TCR loss in normal T cells in vitro. T lymphocytes from RA synovial fluid express less TCR and the linker for the activation of T cells. Although they are well known for their destructive effect on biomolecules, reactive oxygen species are more and more accepted as necessary constituents in signaling pathways and modulators of responses in physiological and pathological conditions. It has been shown that ROS are produced in muscular cells upon binding of ligands such as angiotensin II. In addition, ROS production has been documented in a number of cells stimulated with cytokines such as tumor necrosis factor, transforming growth factor, and interleukin and growth factors such as bovine fibroblast growth factor, nerve growth factor, plateletderived growth factor, and epidermal growth factor inhibited the activation of NFB by phorbol myristate acetate, tumor necrosis factor, and interleukin, strongly supporting the idea that oxygen radicals are implicated in physiological activation processes. Reactive oxygen species trigger several proximal and distal signaling pathways in T lymphocytes, affect the activities of transcription factors, and lead to expression of specific genes. To investigate the molecular basis of oxidative stressinduced T cell hyporesponsiveness, we developed an in vitro system in which T lymphocytes are rendered hyporesponsive by exposure to an oxidative environment generated by activated neutrophils.T cells were cocultured for hwith or without syngenic SF or PB neutrophils in the presence or absence of catalase.Data are expressed in cpm and percentage of control proliferation. Neutrophils were separated from erythrocytes by dextran sedimentation, and residual red blood cells were lysed with icecold.

Leave a Reply